Student in Library

REFEREED JOURNAL ARTICLES

Associate Professor de Graaf has published over 70 peer reviewed research and review articles on a variety of topics in animal reproduction such as production of sexed sperm, cryopreservation of semen, seminal plasma proteomics, transport of sperm through the female tract, artificial insemination and multiple ovulation and embryo transfer. The majority of his work is on sheep, but de Graaf has also published research on rhinos, camels, alpacas, rabbits, and mice.

These articles are listed in chronological order below.

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Full publication listing

  1. Pool, K. R., Rickard, J. P., & de Graaf, S. P. (2021). Overcoming neuroendocrine and metabolic barriers to puberty: the role of melatonin in advancing puberty in ewe lambs. Domestic Animal Endocrinology, 72, 106457.

  2. Pinet-Charvet, C., Fleurot, R., Derouin-Tochon, F., de Graaf, S.P., Druart, X., Tsikis, G., Texeira, A., Labas, V., Moreau, T., Taragnat, C., Cayla, X., and Duittoz, A.H. (2020) beta-Nerve Growth Factor triggers ovulation by stimulating GnRH neurons through a P75 mediated-mechanism. Scientific Reports, 10, 10654

  3. Bernecic, N.C, de Graaf, S.P. Gadella, B.M., and Leahy, T. (2020) Synergism between albumin, bicarbonate and cAMP upregulation for cholesterol efflux from ram sperm. Reproduction, 160 269-280.

  4. Pool, K. R., Pini, T., Rickard, J. P., & de Graaf, S. P. (2020). Exogenous melatonin advances the ram breeding season and increases testicular function. Scientific Reports, 10, 9711

  5. Leahy, T., Rickard, J., Pini, T., Gadella, B. M. and de Graaf, S. P. (2020) Quantitative proteomic analysis of seminal plasma, sperm membrane proteins and seminal extracellular vesicles suggests vesicular mechanisms aid in the removal and addition of proteins to the ram sperm membrane. Proteomics, 20, 1900289.

  6. Pool, K. R., Rickard, J. P., & de Graaf, S. P. (2020). Overcoming neuroendocrine and metabolic barriers to puberty: the role of melatonin in advancing puberty in ewe lambs. Domestic Animal Endocrinology, 72, 106457

  7. Green, C., Rickard, J. P., de Graaf, S. P., & Crean, A. J. (2020). From One Ejaculate to Another: Transference of Sperm Traits via Seminal Plasma Supplementation in the Ram. Biology, 9(2), 33.

  8. Rickard JP and de Graaf SP (2020) Sperm surface changes and their consequences for sperm transit through the female reproductive tract. Theriogenology, 150 96-105.

  9. Al-Bulushi, S., Manjunatha, B.M., Bathgate, R., Rickard, J. and de Graaf, S.P. (2019) Artificial insemination with fresh, liquid stored and frozen thawed semen in dromedary camels. Plos One, 14(11), e0224992

  10. Stuart, C. C., Vaughan, J. L., Kershaw-Young, C. M., de Graaf, S.P., and Bathgate, R. (2019) Effect of diluent type, cryoprotectant concentration, storage method and freeze/thaw rates on the post-thaw quality and fertility of cryopreserved alpaca spermatozoa. Scientific Reports, 9: 12826

  11. Katigbak, R.D., Turchini, G.M., de Graaf, S.P., Lingxue Kong, L. and Dumée, L.F. (2019) Review on Sperm Sorting Technologies and Perspectives in Sperm Properties for New Separation Methods Utilizing the Interface of Biochemistry and Material Science. Advanced Biosystems, 3, 1900079

  12. Al-Bulushi, S., Manjunatha, B.M., Bathgate, R., Rickard, J.P., and de Graaf, S.P. (2019) Liquid storage of dromedary camel semen in different extenders. Animal Reproduction Science, 207 95-106.

  13. Bernecic, N.C., Zhang, M., Gadella, B.M., Brouwers J.F.H.M., Jansen, J.W.A., Arkesteijn, G.J.A., de Graaf, S.P. and Leahy, T. (2019) BODIPY-cholesterol can be reliably used to monitor cholesterol efflux from capacitating mammalian spermatozoa. Scientific Reports, 9, 9804.

  14. Friesen, C.R., de Graaf, S.P., Olsson, M. (2019) The relationship of body condition, superoxide dismutase and superoxide with sperm performance. Behavioural Ecology, 30 1351-1363

  15. Druart, X., Rickard, J.P., Tsikas, G. and de Graaf, S.P. (2019) Seminal plasma proteins as markers of sperm fertility. Theriogenology, 137 30-35.

  16. Bernecic, N.C., Gadella, B.M., Leahy, T. and de Graaf, S.P. (2019) Novel methods to detect capacitation-related changes in spermatozoa. Theriogenology, 137 56-66

  17. Rickard, J.P., Pool, K.R., Druart, X. and de Graaf, S.P. (2019) The fate of spermatozoa in the female reproductive tract: A comparative review. Theriogenology, 137 104-112

  18. Fair, S., Meade, K.G., Reynaud, K., Druart, X. and de Graaf, S.P. (2019) The biological mechanisms regulating sperm selection by the ovine cervix. Reproduction, 158 R1-R13.

  19. Leahy, T., Rickard, J., Bernecic, N., Druart, X., & de Graaf, S. (2019) Ram seminal plasma and its functional proteomic assessment. Reproduction, 157 R243-R256.

  20. Al-Bulushi, S., Manjunatha, B.M., de Graaf, S.P. and Rickard, J.P. (2019) Reproductive seasonality of male dromedary camels. Animal Reproduction Science 202, 10-20.

  21. Al-Bulushi, S., Manjunatha, B.M., Bathgate, R., Rickard, J.P., and de Graaf, S.P. (2018) Effect of semen collection frequency on the semen characteristics of dromedary camels. Animal Reproduction Science 197, 145-153

  22. Pini, T., Leahy, T., and de Graaf, S.P. (2018) Sublethal sperm freezing damage: Manifestations and solutions. Theriogenology 118, 172-181

  23. Druart, X., and de Graaf, S.P. (2018) Seminal plasma proteomes and sperm fertility. Animal Reproduction Science 194 33-40.

  24. Pini, T., Farmer, K., Druart, X., Teixeira-Gomes, A.P., Tsikis, G., Labas, V., Leahy, T., and de Graaf, S.P. (2018) Binder of Sperm Proteins protect ram spermatozoa from freeze-thaw damage. Cryobiology 82 78-87.

  25. Pini, T., Rickard, J.P., Leahy, T., Crossett, B., Druart, X., and de Graaf, S.P. (2018) Cryopreservation and egg yolk medium alter the proteome of ram spermatozoa. Journal of Proteomics 181 73-82.

  26. Pini, T., de Graaf, S.P., Druart, X., Tsikis, G., Labas, V., Teixeira-Gomes, A.P., Gadella, B.M., and Leahy, T. (2018) Binder of Sperm Proteins 1 and 5 have contrasting effects on the capacitation of ram spermatozoa. Biology of Reproduction 98(6) 765-775.

  27. Pini, T., Leahy, T., and de Graaf, S.P. (2018) Seminal plasma and cryopreservation alter ram sperm surface carbohydrates and interactions with neutrophils. Reproduction, Fertility & Development 30 689-702

  28. Rickard, J.P., Ryan, G., Hall, E., de Graaf, S.P., and Hermes, R. (2017) Using transrectal ultrasound to examine the effect of exogenous progesterone on early embryonic loss in sheep. PLOS ONE 12(8), e0183659

  29. Maddison J, Rickard J, Bernecic N, Tsikis G, Soleilhavoup C, Labas V, Combes-Soia L, Harichaux G, Druart X, Leahy T, de Graaf SP (2017) Oestrus synchronisation and superovulation alter the cervicovaginal mucus proteome of the ewe. Journal of Proteomics 155 1-10.

  30. Pini, T., Leahy, T., Soleilhavoup, C., Tsikis, G., Labas, V., Combes-Soia, L., Harichaux, G., Rickard, J.P., Druart, X and de Graaf, S. P. (2016). A proteomic investigation of ram spermatozoa and the proteins conferred by seminal plasma. Journal of Proteome Research 15 3700-3711.

  31. Maddison, J. W., Rickard, J. P., Mooney, E., Bernecic, N. C., Soleilhavoup, C., Tsikis, G., Druart, X., Leahy, T. and de Graaf, S. P. (2016) Oestrus synchronisation and superovulation alter the production and biochemical constituents of ovine cervicovaginal mucus. Animal Reproduction Science, 172, 114-122.

  32. McGregor, B.A., de Graaf, S.P., Hatcher, S. (2016) On-farm factors affecting physical quality of Merino wool. 1. Nutrition, reproduction, health and management. Small Ruminant Research 137 138-150.

  33. Leahy, T., Rickard, J.P., Aitken, R.J., and de Graaf, S.P. (2016) D-penicillamine prevents ram sperm agglutination by reducing the disulphide bonds of a copper-binding sperm protein. Reproduction 151, 491-500.

  34. Rickard, J.P., Schmidt, R.E., Maddison, J.W., Bathgate, R., Lynch, G.W., Druart, X., and de Graaf, S.P. (2016) Variation in seminal plasma alters the ability of ram spermatozoa to survive cryopreservation. Reproduction, Fertility & Development 28(4) 516-523.

  35. Leahy, T., Rickard, J.P., Aitken, R.J., and de Graaf, S.P. (2016) Penicillamine prevents ram sperm agglutination in media that support capacitation. Reproduction 151(2), 167-177

  36. Leahy, T., and de Graaf, S. (2016). The ART of milk production. Australasian Science, 37 (1), 32-34.

  37. Soleilhavoup, C., Riou, C., Tsikis, G., Labas, V., Harichaux, G., Kohnke, P., Reynaud, K., de Graaf, S.P., Gerard, N., and Druart, X. (2015) Proteomes of the female genital tract during the oestrous cycle. Molecular & Cellular Proteomics 15, 93-108.

  38. Mata-Campuzano, M., Soleilhavoup, C., Tsikis, G., Martinez-Pastor, F., de Graaf, S.P., and Druart, X. (2015) Motility of liquid stored ram spermatozoa is altered by dilution rate independent of seminal plasma concentration. Animal Reproduction Science 162, 31-36.

  39. Stuart, C. C., Vaughan, J. L., Kershaw-Young, C. M., Wilkinson, J., Bathgate, R., and de Graaf, S. P. (2015). Effects of varying doses of β-nerve growth factor on the timing of ovulation, plasma progesterone concentration and corpus luteum size in female alpacas (Vicugna pacos). Reproduction, Fertility & Development 27, 1181-1186.

  40. Rickard, J.P., Leahy, T., Soleilhavoup, C., Tsikis, G., Labas, V., Harichaux, G., Lynch, G.W., Druart, X., and de Graaf, S.P. (2015) The identification of proteomic markers of sperm freezing resilience in ram seminal plasma. Journal of Proteomics 126, 303-311.

  41. Johinke, D., de Graaf, S.P., and Bathgate, R. (2015) The effect of sperm concentration and storage vessel on quercetin-supplemented rabbit semen during chilled storage. Reproduction in Domestic Animals 50, 567-573

  42. Johinke, D., de Graaf, S.P., and Bathgate, R. (2014) Quercetin reduces the in vitro production of H2O2 during chilled storage of rabbit spermatozoa. Animal Reproduction Science 151(3-4), 208-19.

  43. Rickard, J.P., Pini, T., Soleilhavoup, C., Cognié, J., Bathgate, R., Lynch, G.W., Evans, G., Maxwell, W.M.C., Druart, X., and de Graaf, S.P. (2014) Seminal plasma aids the survival and cervical transit of epididymal ram spermatozoa. Reproduction 148, 469-478.

  44. Soleilhavoup, C., Tsikis, G., Labas, V., Harichaux, G., Kohnke, P. L., Dacheux, J.L., Guérin, Y., Gatti, J.L., de Graaf, S. P. and Druart, X. (2014) Ram seminal plasma proteome and its impact on liquid preservation of spermatozoa. Journal of Proteomics, 109(23), 245-260.

  45. de Graaf, S.P., Leahy, T. and Vishwanath, V. (2014) Biological and practical lessons associated with the use of sexed semen. Reproduction in Domestic Ruminants VIII, 507-522 (invited review).

  46. de Graaf, S.P., Rickard, J.P., Pini, T., Maddison, J.W., Druart, X., and Leahy, T. (2014) Emerging roles of seminal plasma in sperm function. Proc Assoc Applied Animal Andrology, 8th – 10th August, Newcastle, Australia (invited review). pp 93-102.

  47. Johinke, D., de Graaf, S.P., Bathgate, R. (2014). Investigation of in vitro parameters and in vivo fertility of rabbit spermatozoa after chilled storage. Animal Reproduction Science, 147(3-4), 135-143.

  48. Garratt, M., Bathgate, R., de Graaf, S.P., Brooks, R. C. (2013) Copper-zinc superoxide dismutase deficiency impairs sperm motility and in vivo fertility. Reproduction 146, 297-304.

  49. Druart, X., Rickard, J.P., Mactier, S., Kohnke, P.L., Kershaw-Young, C.M., Bathgate, R., Gibb, Z., Crossett, B., Tsikis, G., Labas, V., Harichaux, G., Grupen, C.G., and de Graaf, S.P. (2013) Proteomic characterization and cross species comparison of mammalian seminal plasma. Journal of Proteomics 91, 13-22.

  50. Bathgate, R., Mace, N., Heasman, K., Evans, G., Maxwell, W.M.C., and de Graaf, S.P. (2013) Birth of Kids After Artificial Insemination with Sex-Sorted, Frozen-Thawed Goat Spermatozoa. Reproduction in Domestic Animals 48(6), 893-8.

  51. Rath, D., Barcikowski, S., de Graaf, S.P., Garrels, W., Grossfeld, R., Klein, S., Knabe, W., Knorr, C., Kues, W., Meyer, H., Michl, J., Moench-Tegeder, G., Rehbock, C., Taylor, U., and Washausen, S. (2013) Sex selection of sperm in farm animals: status report and developmental prospects. Reproduction 145(1), R15-R30

  52. Portas, T., Evans, G., and de Graaf, S.P. (2012) The seminiferous cycle of the rhinoceros. Animal Reproduction Science 136(1–2), 128-132

  53. Leahy T. and de Graaf S. P (2012) Seminal plasma and its effect on ruminant spermatozoa during processing. Reproduction in Domestic Animals, 47 (s4) 207-213 (invited review).

  54. Beilby K. H., de Graaf S. P., Evans G., Maxwell W. M. C., Wilkening S., Wrenzycki C. and Grupen C. G. (2011) Quantitative mRNA Expression in Ovine Blastocysts Produced from X- and Y-Chromosome Bearing Sperm, Both In Vitro and In Vivo. Theriogenology, 76 (3) 471-481.

  55. Beilby K. H., de Graaf S. P. and Grupen C. G. (2010) The effect of sperm and cryoprotectant concentration on the freezing success of sex-sorted ram sperm for in vitro fertilisation. Theriogenology 74 (5) 786-794.

  56. de Graaf, S. P., Druart, X. (2010) Interaction of spermatozoa with the female reproductive tract. Proc Assoc Applied Animal Andrology, 28th – 29th August, Sydney, Australia (invited review).

  57. de Graaf, S. P., Druart, X. (2010) Sperm interaction with the female reproductive tract. Proc Australian College of Vet Scientists, July 1st – 3rd, Surfers Paradise, QLD, Australia (invited review).

  58. Evans, G. and de Graaf, S. P. (2010) Conservation and application of sexed sperm in breeding programs. International Strategic Programs for the Conservation of Animal Genetic Resources for Food and Agriculture, July 17th, 2010, Vancouver, British Columbia, Canada pp 84-91 (invited review).

  59. Behr, B., Rath, D., Hildebrandt, T. B., Göritz, F., Blottner, S., Portas, T. J., Bryant, B. R., Sieg, B., Knieriem, A., de Graaf, S. P., Maxwell, W. M. C., and Hermes, R. (2009). Germany/Australia Index of sperm sex sortability in elephants and rhinoceros. Reproduction in Domestic Animals, 44 (2), 273-277.

  60. Behr B., Rath D., Mueller P., Hildebrandt T. B., Goeritz F., Braun B. C., Leahy T., de Graaf S. P., Maxwell W. M. C. and Hermes R. (2009) Feasibility of sex-sorting sperm from the white and the black rhinoceros (Ceratotherium simum, Diceros bicornis). Theriogenology, 72, 353-364.

  61. de Graaf S. P., Beilby K. H., Underwood S. L., Evans G. and Maxwell W. M. C. (2009) Sperm sexing in sheep and cattle: The exception and the rule. Theriogenology, 71 (1), 89-97 (invited review).

  62. de Graaf, S. P., Maxwell, W. M. C. and Evans, G. (2008) Functional differences between sex-sorted and non-sorted sperm. Proc Assoc Applied Animal Andrology, 12th – 13th July, Budapest, Hungary (invited review).

  63. de Graaf S. P., Leahy T., Marti J. I., Evans G. and Maxwell W. M. C. (2008) Application of seminal plasma in sex-sorting and sperm cryopreservation. Theriogenology, 70, 1360-1363 (invited review).

  64. de Graaf, S. P., Beilby, K. H., O'Brien, J. K., Osborn, D., Downing, J. A., Maxwell, W. M. C., and Evans, G. (2007). Embryo production from superovulated sheep inseminated with sex-sorted ram spermatozoa. Theriogenology, 67 (3), 550-555.

  65. de Graaf, S. P., Evans, G., Gillan, L., Guerra, M. M. P., Maxwell, W. M. C., and O'Brien, J. K. (2007). The influence of antioxidant, cholesterol and seminal plasma on the in vitro quality of sorted and non-sorted ram spermatozoa. Theriogenology, 67 (2), 217-227.

  66. de Graaf, S. P., Evans, G., Maxwell, W. M. C., Cran, D. G., and O'Brien, J. K. (2007). Birth of offspring of pre-determined sex after artificial insemination of frozen-thawed, sex-sorted and re-frozen-thawed ram spermatozoa. Theriogenology, 67 (2), 391-398.

  67. de Graaf, S. P., Evans, G., Maxwell, W. M. C., Downing, J. A., and O'Brien, J. K. (2007). Successful low dose insemination of flow cytometrically sorted ram spermatozoa in sheep. Reproduction in Domestic Animals, 42, 648-653.

  68. de Graaf, S. P., Peake, K., Maxwell, W. M. C., O'Brien, J. K., and Evans, G. (2007). Influence of supplementing diet with oleic and linoleic acid on the freezing ability and sex-sorting parameters of ram semen. Livestock Science, 110 (1-2), 166-173.

  69. de Graaf, S. P., Evans, G., Maxwell, W. M. C., and O'Brien, J. K. (2006). In vitro function of fresh and frozen-thawed ram spermatozoa after sex-sorting and re-freezing. Reproduction, Fertility, & Development, 18 (8), 867-874.

  70. Maxwell, W. M. C., de Graaf, S. P., El-Hajj Ghaoui, R., and Evans, G. (2006). Seminal plasma effects on sperm handling and female fertility. Society for Reproduction and Fertility Supplement, 64, 13-38 (invited review).

  71. Morton, K. M., de Graaf, S. P., Campbell, A., Tomkins, L. M., Maxwell, W. M. C. and Evans, G. (2005) Repeat ovum pick-up and in vitro embryo production from adult ewes with and without FSH treatment. Reproduction in Domestic Animals, 40 (5), 422-428.

  72. Maxwell, W. M. C., Evans, G., Hollinshead, F.K., Bathgate, R., de Graaf, S. P., Eriksson, B. M., Gillan, L., Morton, K. M. and O'Brien, J. K. (2004) Integration of sperm sexing technology into the ART toolbox. Animal Reproduction Science, 82-83, 79-95 (invited review).